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Virtual poster session

Keratinocytes hacat proliferation on 3D-printed chitosan-collagen scaffolds

Wednesday (07.10.2020)
19:31 - 19:34 Room 1
Part of:
Line-Up:
18:40 Virtual poster session Development of CFRP - hybrid foam composites for improved light-weight design of electric vehicles 1 Joachim Baumeister
18:43 Virtual poster session Study of the thermal conductivity of powdered microcellular polymers based on PMMA 1 Ph.D. Ismael Sánchez
18:46 Virtual poster session Manufacturing of functional aluminium matrix syntactic foams 1 Alexandra Kemény
18:49 Virtual poster session Manufacturing and comparison of AlSi12 matrix unimodal and bimodal syntactic metal foams 1 Borbála Leveles
18:52 Virtual poster session The effect of elastomer viscosity in pp/poe foams produced by core-back injection moulding over impact properties 1 Ph.D. Santiago Muñoz Pascual
18:55 Virtual poster session Modelling of the mechanisms of heat transfer in glass foams at different temperatures 1 Paula Cimavilla-Román
18:58 Virtual poster session Thermal conductivity of polyisocyanurate foams (PIR) produced using hydrofluoroolefins (HFOs) as blowing agents 1 Ph.D. Patricia Torres
19:01 Virtual poster session Multiscale modeling of biopolymer aerogels 1 Dr.-Ing. Ameya Rege
19:04 Virtual poster session On the way to mass production of unit-cell based auxetic materials 1 Angela Schwarz
19:07 Virtual poster session Wavy knits – enabling the production of auxetic materials 1 Angela Schwarz
19:10 Virtual poster session Influence of Sample Size on Dynamic Compression of Metal Syntactic Foams 1 Nima Movahedi
19:13 Virtual poster session Synthesis of new non-isocyanate polyurethane foams based on cyclic triscarbonates and diamines 1 Ph.D. Mercedes Santiago-Calvo
19:16 Virtual poster session Formation of monodisperse surfactant foams 1 Prof. Dr. Rumen Krastev
19:19 Virtual poster session Analysis of a AlMg2.5 matrix syntactic foam produced via thixoinfiltration of loosely packed and micro structured recycled balloons 1 João Paulo Paschoal
19:22 Virtual poster session In-situ observation of combustion foaming process for synthesizing porous Al3Ti by X-ray radioscopy 1 Takamasa Inukai
19:25 Virtual poster session Characterization of 3D printed PHBV+BG scaffolds for bone regeneration 1 Prof. Dr. Elida B. Hermida
19:28 Virtual poster session Rheology of a hydrogel-ink, printing fidelity and elastic behavior of 3D-printed scaffolds 1 Prof. Dr. Elida B. Hermida
19:31 Virtual poster session Keratinocytes hacat proliferation on 3D-printed chitosan-collagen scaffolds 1 Prof. Dr. Elida B. Hermida
19:34 Virtual poster session The microhardness and microstructure studies on the sintered open-cell nickel foam 0 Faeze Barzegar
19:37 Virtual poster session Characterization of porous titanium implants produced by 3D printer technique 0 Dr. Akram Salehi
19:40 Virtual poster session Adapting polymer derived ceramic membranes for emulsification process 1 Natália Cristina Fontão
19:43 Virtual poster session Poster discussion in video chat rooms 0
Session Chair


Session V.1: Session 1
Belongs to:
General Topic V: Virtual poster session


Despite the wide advances in biomaterials for tissue engineering, autografts are today the only artifact to promote epidermis regeneration in chronic wounds and in acute wounds greater than 4 cm in diameter. This feature remarks the importance of autologous epidermal cells in the healing process, thus the importance of developing biomaterials for seeding these cells. Hydrogels have largely proved to be suitable for cells proliferation, and 3D bioprinting has become an innovative technique for setting biomaterials and cells according to a controlled digital design. We aimed to inquire in the collagen (Col)) and chitosan (Chit) blends to this type of application.

Two printable hydrogel precursors were selected, in % w/v, Chit 1.00:Col 0.36 and Chit 0.50:Col 0.54, both at pH=4.50 because of the polymer solubilities. They were analyzed rheologically, particularly considering the strain rate of a 3D-bioprinter (30-60 1/s) made in Argentina.Viscosities during the extrusion of the hydrogel were in the interval 0.5-0.8 Pa.s. In order to make the inks suitable for cell life, a method for misting NaHCO3 0.8 M was developed, obtaining pH ~ 6 homogenous inks storable during 5 days with neglegible changes in stability, viscosity and printing fidelity, when compared to their non-misted counterparts.

To achieve proper mechanical properties, activators EDC/ NHS in powder were added to the hydrogels immediately before printing. The final products were crosslinked manipulable scaffolds, not cytotoxic according to the ISO rule 10993-5 using NH-3T3 fibroblasts. In order to advance towards a possible epidermis regeneration, keratinocytes proliferation was tested by seeding 105 HaCat cells/well, in a 24-wells plate with a printed scaffold in each well. Cells showed excellent adhesion and capability to proliferate on the hydrogels after 5 days in both scaffolds. Window time available for printing without gelation remains to be defined, as well as scaffold swelling and stability, both features partially dimmable by crosslinkers concentration.

Speaker:
Prof. Dr. Elida B. Hermida
National University of San Martin - UNSAM
Additional Authors:
  • Dr. Mercedes Pérez Recalde
    National University of San Martin
  • Julieta Polenta
    National University of San Martin
  • Dr. Ana González Wusener
    CONICET-UNSAM
  • A. Bolgiani
    German Hospital of Buenos Aires, Burns Service, Argentina
  • Ph.D. Mercedes Pérez-Recalde
    National University of San Martin - UNSAM

Dateien

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